Application
WB analysis of HeLa lysate and H3 protein using Histone H3K27me1 (mono-methyl Lys27) antibody.Application
IHC-P analysis of rat brain tissue using Histone H3K27me1 (mono-methyl Lys27) antibody at a dilution of 1:200.Application
IHC-P analysis of human thyroid cancer tissue using Histone H3K27me1 (mono-methyl Lys27) antibody at a dilution of 1:200.Application
IHC-P analysis of rat lung tissue using Histone H3K27me1 (mono-methyl Lys27) antibody at a dilution of 1:200.Application
IHC-P analysis of human kidney cancer tissue using Histone H3K27me1 (mono-methyl Lys27) antibody at a dilution of 1:200.Application
ICC/IF analysis of 293T cells using Histone H3K27me1 (mono-methyl Lys27) antibody (red) and DAPI (blue).Application
Dot-blot analysis of all sorts of methylation peptides using Histone H3K27me1 (mono-methyl Lys27) antibody.| Product Name | Histone H3K27me1 (mono-methyl Lys27) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3K27me1 (mono-methyl Lys27)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
| Modification | mono-methyl Lys27 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | Dot,ICC/IF,IHC-P,IP,WB,ChIP |
| WB 1:500 - 1:2000. IHC 1:50 - 1:200. IF 1:50 - 1:200. IP 1:50 - 1:200. ChIP 1:20 - 1:100. CHIPseq 1:20 - 1:100. | |
| Species Reactivity | Human,Mouse,Other |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HIST3H3 , H3.4 , histone cluster 3, H3 , H3FT , H3t , H3/g |
|---|---|
| Target | Histone H3K27me1 (mono-methyl Lys27) |
| Molecular Weight(MW) | 15 kDa (note) |
Application
WB analysis of HeLa lysate and H3 protein using Histone H3K27me1 (mono-methyl Lys27) antibody.
Application
IHC-P analysis of rat brain tissue using Histone H3K27me1 (mono-methyl Lys27) antibody at a dilution of 1:200.
Application
IHC-P analysis of human thyroid cancer tissue using Histone H3K27me1 (mono-methyl Lys27) antibody at a dilution of 1:200.
Application
IHC-P analysis of rat lung tissue using Histone H3K27me1 (mono-methyl Lys27) antibody at a dilution of 1:200.
Application
IHC-P analysis of human kidney cancer tissue using Histone H3K27me1 (mono-methyl Lys27) antibody at a dilution of 1:200.
Application
ICC/IF analysis of 293T cells using Histone H3K27me1 (mono-methyl Lys27) antibody (red) and DAPI (blue).
Application
Dot-blot analysis of all sorts of methylation peptides using Histone H3K27me1 (mono-methyl Lys27) antibody.| Application Notes | WB 1:500 - 1:2000. IHC 1:50 - 1:200. IF 1:50 - 1:200. IP 1:50 - 1:200. ChIP 1:20 - 1:100. CHIPseq 1:20 - 1:100. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
