Application
ChIP was performed with HeLa nuclear extract and either 2 μg of H3K27me2 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MyoD1 or GAPDH promoter.Application
Dot blot analysis of peptides containing modified histone H3 and H4 and the unmodified sequence using H3K27me2 antibody at a dilution of 1:50,000. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane for analysis.Application
ICC/IF analysis of HeLa cells using H3K27me2 antibody and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27me2 antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).Application
WB analysis of histone extracts (15 μg) from HeLa cells using H3K27me2 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.Application
ELISA was performed using a serial dilution of Histone H3K27me2 (di-Methyl Lys27) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:480,000.| Product Name | Histone H3K27me2 (dimethyl Lys27) antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H3K27me2 (dimethyl Lys27) |
| Modification | dimethyl Lys27 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | Dot,ELISA,ICC/IF,WB,ChIP |
| ChIP (5 µg/ChIP), ELISA (1:100 - 1:500), Dot blotting (1:20,000), Western blotting (1:1,000) | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Histone H3 di Methyl, di Methyl Histone H3, histone cluster 1, H3a, Histone H3 di-Methyl K27, H3/A, HIST1H3A, diMethyl Lys27 Histone H3, Histone H3 di-Methyl Lys27, Histone H3 di-Methyl, di Methyl K27 Histone H3, H3FA, di Methyl Histone H3 K27, diMethyl K2 |
|---|---|
| Target | Histone H3 (di-Methyl Lys27) |
Application
ChIP was performed with HeLa nuclear extract and either 2 μg of H3K27me2 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MyoD1 or GAPDH promoter.
Application
Dot blot analysis of peptides containing modified histone H3 and H4 and the unmodified sequence using H3K27me2 antibody at a dilution of 1:50,000. One hundred to 0.2 pmol of peptide containing the respective histone modification were spotted on a membrane for analysis.
Application
ICC/IF analysis of HeLa cells using H3K27me2 antibody and DAPI. Cells were fixed with 4% formaldehyde for 10’ and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H3K27me2 antibody at a dilution of 1:500 (green). The nuclei were stained with DAPI (blue).
Application
WB analysis of histone extracts (15 μg) from HeLa cells using H3K27me2 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.
Application
ELISA was performed using a serial dilution of Histone H3K27me2 (di-Methyl Lys27) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:480,000.| Application Notes | ChIP (5 µg/ChIP), ELISA (1:100 - 1:500), Dot blotting (1:20,000), Western blotting (1:1,000) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
