Application
WB analysis of HeLa lysate and H3 protein using Histone H3K27me3 (tri-methyl Lys27) antibody.Application
IHC-P analysis of rat testis tissue using Histone H3K27me3 (tri-methyl Lys27) antibody at a dilution of 1:200.Application
IHC-P analysis of rat brain tissue using Histone H3K27me3 (tri-methyl Lys27) antibody at a dilution of 1:200.Application
IHC-P analysis of human thyroid cancer tissue using Histone H3K27me3 (tri-methyl Lys27) antibody at a dilution of 1:200.Application
IHC-P analysis of human rectal cancer tissue using Histone H3K27me3 (tri-methyl Lys27) antibody at a dilution of 1:200.Application
ICC/IF analysis of 293T cells using Histone H3K27me3 (tri-methyl Lys27) antibody (red) and DAPI (blue).Application
ChIP assay was performed with 293 lysate and either Histone H3K27me3 (tri-methyl Lys27) antibody or rabbit IgG. The precipitated DNA was detected by qPCR with primer sets targeting to indicated genes. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.Application
Dot-blot analysis of all sorts of methylation peptides using Histone H3K27me3 (tri-methyl Lys27) antibody.| Product Name | Histone H3K27me3 (tri-methyl Lys27) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3K27me3 (tri-methyl Lys27)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3 |
| Modification | tri-methyl Lys27 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | Dot,ICC/IF,IHC-P,IP,WB,ChIP |
| WB: 1:500 - 1:200. IHC: 1:50 - 1:20. IF: 1:50 - 1:20. IP: 1:50 - 1:20. ChIP: 1:20 - 1:100 | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H3FT , histone cluster 3, H3 , H3.4 , HIST3H3 , H3/g , H3t |
|---|---|
| Target | Histone H3K27me3 (tri-methyl Lys27) |
| Molecular Weight(MW) | 15 kDa (note) |
Application
WB analysis of HeLa lysate and H3 protein using Histone H3K27me3 (tri-methyl Lys27) antibody.
Application
IHC-P analysis of rat testis tissue using Histone H3K27me3 (tri-methyl Lys27) antibody at a dilution of 1:200.
Application
IHC-P analysis of rat brain tissue using Histone H3K27me3 (tri-methyl Lys27) antibody at a dilution of 1:200.
Application
IHC-P analysis of human thyroid cancer tissue using Histone H3K27me3 (tri-methyl Lys27) antibody at a dilution of 1:200.
Application
IHC-P analysis of human rectal cancer tissue using Histone H3K27me3 (tri-methyl Lys27) antibody at a dilution of 1:200.
Application
ICC/IF analysis of 293T cells using Histone H3K27me3 (tri-methyl Lys27) antibody (red) and DAPI (blue).
Application
ChIP assay was performed with 293 lysate and either Histone H3K27me3 (tri-methyl Lys27) antibody or rabbit IgG. The precipitated DNA was detected by qPCR with primer sets targeting to indicated genes. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Application
Dot-blot analysis of all sorts of methylation peptides using Histone H3K27me3 (tri-methyl Lys27) antibody.| Application Notes | WB: 1:500 - 1:200. IHC: 1:50 - 1:20. IF: 1:50 - 1:20. IP: 1:50 - 1:20. ChIP: 1:20 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
