Application
ChIP was performed with U2OS nuclear extract and either 1 μg of H3K36me1 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MyoD or GAPDH.Application
Dot blot analysis of peptides containing other modifications of histone H3 or the unmodified H3K36 using H3K36me1 antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane for analysis.Application
ICC/IF analysis of U2OS cells using H3K36me1 antibody and DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. The cells were immunofluorescently labeled with the H3K36me1 antibody at a dilution of 1:500 (red). The nucleu were stained with DAPI (blue).Application
ICC/IF analysis of U2OS cells using H3K36 unmodified peptide-blocked H3K36me1 antibody and DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. The cells were immunofluorescently labeled with the H3K36 unmodified peptide-blocked H3K36me1 antibody at a dilution of 1:500 (red). The nucleu were stained with DAPI (blue).Application
ICC/IF analysis of U2OS cells using H3K36me1 peptide-blocked H3K36me1 antibody and DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. The cells were immunofluorescently labeled with the H3K36me1 peptide-blocked H3K36me1 antibody at a dilution of 1:500 (red). The nucleu were stained with DAPI (blue).Application
ICC/IF analysis of U2OS cells using H3K36me2 peptide-blocked H3K36me1 antibody and DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. The cells were immunofluorescently labeled with the H3K36me2 peptide-blocked H3K36me1 antibody at a dilution of 1:500 (red). The nucleu were stained with DAPI (blue).Application
ICC/IF analysis of U2OS cells using H3K36me3 peptide-blocked H3K36me1 antibody and DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. The cells were immunofluorescently labeled with the H3K36me3 peptide-blocked H3K36me1 antibody at a dilution of 1:500 (red). The nucleu were stained with DAPI (blue).Application
WB analysis of histone extracts (15 μg) from HeLa cells using H3K36me1 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.Application
ELISA was performed using a serial dilution of Histone H3K36me1 (mono-Methyl Lys36) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:46,000.WB
C Assays of histone methylation patterns using Western blot. Histone H3 was used as the loading control.WB
c Analysis of H3K4 and H3K36 methylation patterns through Western blot.| Product Name | Histone H3K36me1 (monomethyl Lys36) antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H3K36me1 (monomethyl Lys36) |
| Modification | monomethyl Lys36 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| ChIP (5-10 µg), ELISA (1:100), Dot blotting (1:20,000 ), Western blotting (1:1,000) | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Histone H3 mono-Methyl K36, histone cluster 1, H3a, mono Methyl Lys36 Histone H3, H3/A, HIST1H3A, mono Methyl Histone H3 Lys36, monoMethyl Lys36 Histone H3, mono Methyl Histone H3, Histone H3 monoMethyl, mono-Methyl K36 Histone H3, Histone H3 mono-Methyl L |
|---|---|
| Target | Histone H3 (mono-Methyl Lys36) |
Application
ChIP was performed with U2OS nuclear extract and either 1 μg of H3K36me1 antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to MyoD or GAPDH.
Application
Dot blot analysis of peptides containing other modifications of histone H3 or the unmodified H3K36 using H3K36me1 antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane for analysis.
Application
ICC/IF analysis of U2OS cells using H3K36me1 antibody and DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. The cells were immunofluorescently labeled with the H3K36me1 antibody at a dilution of 1:500 (red). The nucleu were stained with DAPI (blue).
Application
ICC/IF analysis of U2OS cells using H3K36 unmodified peptide-blocked H3K36me1 antibody and DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. The cells were immunofluorescently labeled with the H3K36 unmodified peptide-blocked H3K36me1 antibody at a dilution of 1:500 (red). The nucleu were stained with DAPI (blue).
Application
ICC/IF analysis of U2OS cells using H3K36me1 peptide-blocked H3K36me1 antibody and DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. The cells were immunofluorescently labeled with the H3K36me1 peptide-blocked H3K36me1 antibody at a dilution of 1:500 (red). The nucleu were stained with DAPI (blue).
Application
ICC/IF analysis of U2OS cells using H3K36me2 peptide-blocked H3K36me1 antibody and DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. The cells were immunofluorescently labeled with the H3K36me2 peptide-blocked H3K36me1 antibody at a dilution of 1:500 (red). The nucleu were stained with DAPI (blue).
Application
ICC/IF analysis of U2OS cells using H3K36me3 peptide-blocked H3K36me1 antibody and DAPI. Cells were fixed with 4% formaldehyde for 20’ and blocked with PBS/TX-100 containing 5% normal goat serum. The cells were immunofluorescently labeled with the H3K36me3 peptide-blocked H3K36me1 antibody at a dilution of 1:500 (red). The nucleu were stained with DAPI (blue).
Application
WB analysis of histone extracts (15 μg) from HeLa cells using H3K36me1 antibody diluted 1:1,000 in TBS-Tween containing 5% skimmed milk.
Application
ELISA was performed using a serial dilution of Histone H3K36me1 (mono-Methyl Lys36) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:46,000.
WB
C Assays of histone methylation patterns using Western blot. Histone H3 was used as the loading control.
WB
c Analysis of H3K4 and H3K36 methylation patterns through Western blot.| Application Notes | ChIP (5-10 µg), ELISA (1:100), Dot blotting (1:20,000 ), Western blotting (1:1,000) |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
