Application
Histone H3 (di-methyl Lys4) antibody immunoprecipitates Histone H3 (di-methyl Lys4) protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Histone H3 (di-methyl Lys4) protein by 2 μg of Histone H3 (di-methyl Lys4) antibody 15% SDS-PAGE The immunoprecipitated Histone H3 (di-methyl Lys4) protein was detected by Histone H3 (di-methyl Lys4) antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE diluted at 1:5000Application
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3K4me2 (dimethyl Lys4) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or β-globin promoter.Application
Histone H3K4me2 (dimethyl Lys4) antibody detects Histone H3K4me2 (dimethyl Lys4) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K4me2 (dimethyl Lys4) protein stained by Histone H3K4me2 (dimethyl Lys4) antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Application
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Histone H3 (di-Methyl K4) antibody at 1:250 dilution.Application
Histone H3K4me2 (dimethyl Lys4) antibody detects Histone H3K4me2 (dimethyl Lys4) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K4me2 (dimethyl Lys4) protein stained by Histone H3K4me2 (dimethyl Lys4) antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.| Product Name | Histone H3K4me2 (dimethyl Lys4) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3 (di-Methyl Lys4) (histone cluster 1, H3a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Modification | dimethyl Lys4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF,IHC-P,IP,WB,ChIP |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, ChimpanzeeMouse (100%), Chimpanzee (100%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H3/A antibody, H3FA antibody, HIST1H3B antibody, HIST1H3C antibody, HIST1H3D antibody, HIST1H3E antibody, HIST1H3F antibody, HIST1H3G antibody, HIST1H3H antibody, HIST1H3I antibody, HIST1H3J antibody, HIST1H3A antibody, histone H3/j antibody, |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H3 (di-methyl Lys4) antibody immunoprecipitates Histone H3 (di-methyl Lys4) protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of Histone H3 (di-methyl Lys4) protein by 2 μg of Histone H3 (di-methyl Lys4) antibody 15% SDS-PAGE The immunoprecipitated Histone H3 (di-methyl Lys4) protein was detected by Histone H3 (di-methyl Lys4) antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE diluted at 1:5000
Application
Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control rabbit IgG or anti-Histone H3K4me2 (dimethyl Lys4) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or β-globin promoter.
Application
Histone H3K4me2 (dimethyl Lys4) antibody detects Histone H3K4me2 (dimethyl Lys4) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K4me2 (dimethyl Lys4) protein stained by Histone H3K4me2 (dimethyl Lys4) antibody diluted at 1:1000. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Application
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using Histone H3 (di-Methyl K4) antibody at 1:250 dilution.
Application
Histone H3K4me2 (dimethyl Lys4) antibody detects Histone H3K4me2 (dimethyl Lys4) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K4me2 (dimethyl Lys4) protein stained by Histone H3K4me2 (dimethyl Lys4) antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
