Application
Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K79me1 antibody.Application
Dot-blot analysis of all sorts of methylation peptides using H3K79me1 antibody.Application
Immunofluorescence analysis of 293T cell using H3K79me1 antibody. Blue: DAPI for nuclear staining.Application
Chromatin Immunoprecipitation analysis extracts of 293 cell line, using MonoMethyl-Histone H3-K79 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.WB
C Assays of histone methylation patterns using Western blot. Histone H3 was used as the loading control.| Product Name | Histone H3K79me1 (mono-methyl Lys79) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3K79me1 (mono-methyl Lys79)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthetic methylated peptide corresponding to residues surrounding K79 of human histone H3 |
| Modification | mono-methyl Lys79 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP assay: 1:20 - 1:100, CHIPseq: 1:20 - 1:100 | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H3.4 , histone cluster 3, H3 , H3FT , H3/g , H3t , HIST3H3 |
|---|---|
| Target | Histone H3K79me1 (mono-methyl Lys79) |
Application
Western blot analysis of extracts of HeLa cell line and H3 protein expressed in E.coli., using H3K79me1 antibody.
Application
Dot-blot analysis of all sorts of methylation peptides using H3K79me1 antibody.
Application
Immunofluorescence analysis of 293T cell using H3K79me1 antibody. Blue: DAPI for nuclear staining.
Application
Chromatin Immunoprecipitation analysis extracts of 293 cell line, using MonoMethyl-Histone H3-K79 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
WB
C Assays of histone methylation patterns using Western blot. Histone H3 was used as the loading control.| Application Notes | WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200, IP: 1:50 - 1:200, ChIP assay: 1:20 - 1:100, CHIPseq: 1:20 - 1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
