Application
Histone H3K9ac (acetyl Lys9) antibody [GT464] detects Histone H3K9ac (acetyl Lys9) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K9ac (acetyl Lys9) protein stained by Histone H3K9ac (acetyl Lys9) antibody [GT464] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Histone H3K9ac (acetyl Lys9) antibody [GT464] detects Histone H3K9ac (acetyl Lys9) protein by western blot analysis. A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 μg HeLa whole cell lysate/extract (0.4 μM Thicostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H3K9ac (acetyl Lys9) antibody [GT464] dilution: 1:5000| Product Name | Histone H3K9ac (acetyl Lys9) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT464] to Histone H3 (acetyl Lys9) (histone cluster 1, H3a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Modification | acetyl Lys9 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT464 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, ChimpanzeeMouse (100%), Chimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | acetyl Lys9 Histone H3 histone cluster 1 H3a acetyl Histone H3 H3/A HIST1H3A acetyl K9 Histone H3 Histone H3 acetyl K9 Histone H3 acetyl Lys9 Histone H3 acetyl H3FA |
|---|---|
| Target | Histone H3 (acetyl Lys9) |
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H3K9ac (acetyl Lys9) antibody [GT464] detects Histone H3K9ac (acetyl Lys9) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K9ac (acetyl Lys9) protein stained by Histone H3K9ac (acetyl Lys9) antibody [GT464] diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Histone H3K9ac (acetyl Lys9) antibody [GT464] detects Histone H3K9ac (acetyl Lys9) protein by western blot analysis. A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 μg HeLa whole cell lysate/extract (0.4 μM Thicostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H3K9ac (acetyl Lys9) antibody [GT464] dilution: 1:5000| Positive Control | HeLa (0.012% DMSO treatment for 18 hr) , HeLa (0.4 µM Thicostatin(TSA) treatment for 18 hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
