Application
Histone H3K9K14ac (acetyl Lys9/Lys14) antibody detects Histone H3K9K14ac (acetyl Lys9/Lys14) protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H3K9K14ac (acetyl Lys9/Lys14) antibody diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Histone H3K9K14ac (acetyl Lys9/Lys14) antibody detects Histone H3K9K14ac (acetyl Lys9/Lys14) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K9K14ac (acetyl Lys9/Lys14) protein stained by Histone H3K9K14ac (acetyl Lys9/Lys14) antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.Application
Histone H3 (acetyl Lys 9, Lys14) antibody detects Histone H3 (acetyl Lys 9, Lys14) protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated) B. 30 µg HeLa whole cell lysate/extract (0.4 µM Trichostatin treatment for 18 hr) 15% SDS-PAGE Histone H3 (acetyl Lys 9, Lys14) antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Dotblot analysis of anti-Histone H3 (acetyl Lys 9, Lys14) antibody with peptide samples. Peptide samples (0.1 µg) were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys 9, Lys14) antibody at different dilution as indicated. A: Peptide samples of Histone H3.1 B: Peptide samples of Histone H3 (acetyl Lys 9, Lys14)| Product Name | Histone H3K9K14ac (acetyl Lys9/Lys14) antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H3 (acetyl Lys10, Lys15) (histone cluster 1, H3a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Modification | acetyl Lys9/Lys14 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | ChimpanzeeChimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H3a antibody histone H3/d antibody histone H3/i antibody histone H3/h antibody member A antibody histone H3/j antibody acetyl Histone H3 antibody histone H3/c antibody HIST1H3A antibody HIST1H3C antibody histone H3.1 antibody histone 1 antibody |
|---|---|
| Molecular Weight(MW) | 15 kDa (note) |
| Cellular Localization | Nucleus |
Application
Histone H3K9K14ac (acetyl Lys9/Lys14) antibody detects Histone H3K9K14ac (acetyl Lys9/Lys14) protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H3K9K14ac (acetyl Lys9/Lys14) antibody diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Histone H3K9K14ac (acetyl Lys9/Lys14) antibody detects Histone H3K9K14ac (acetyl Lys9/Lys14) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H3K9K14ac (acetyl Lys9/Lys14) protein stained by Histone H3K9K14ac (acetyl Lys9/Lys14) antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Hoechst 33342 staining.
Application
Histone H3 (acetyl Lys 9, Lys14) antibody detects Histone H3 (acetyl Lys 9, Lys14) protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated) B. 30 µg HeLa whole cell lysate/extract (0.4 µM Trichostatin treatment for 18 hr) 15% SDS-PAGE Histone H3 (acetyl Lys 9, Lys14) antibody dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Dotblot analysis of anti-Histone H3 (acetyl Lys 9, Lys14) antibody with peptide samples. Peptide samples (0.1 µg) were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys 9, Lys14) antibody at different dilution as indicated. A: Peptide samples of Histone H3.1 B: Peptide samples of Histone H3 (acetyl Lys 9, Lys14)| Positive Control | 293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , HeLa (0.4 μM Trichostatin treatment for 18 hr) |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
