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Histone H3R17me2 (Asymmetric dimethyl Arg17) antibody

Catalog Number:OM256890
OM256890
Discount Price
OM256890-100ul
1~2 Week
100ul
OM256890-50ul
50ul
OM256890-20ul
20ul
1~2 Week
Product Profile
Product Name Histone H3R17me2 (Asymmetric dimethyl Arg17) antibody
Antibody Type Primary Antibodies
Product description Rabbit Polyclonal antibody to Histone3 (di-methyl Arg17) (histone cluster 1, H3a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]
Modification Asymmetric dimethyl Arg17
Key Feature
Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications DotIHC-PWB
*Optimal dilutions/concentrations should be determined by the researcher. :
Species Reactivity HumanMouse
Predicted Reactivity ChimpanzeeChimpanzee (100%)
Concentration 1mg/ml
Purification Affinity purified
Target Information
Gene Synonyms histone cluster 1 antibody
methyl Histone H3 antibody
H3a antibody
histone H3/d antibody
histone H3/i antibody
histone H3/h antibody
histone H3/j antibody
Di methyl Histone H3 antibody
histone H3/c antibody
member A antibody
HIST1H3A antibody
HI
Target Histone H3
Molecular Weight(MW) 15 kDa (note)
Cellular Localization Nucleus
Application

Application

Histone H3R17me2 (dimethyl Arg17) antibody detects Histone H3R17me2 (dimethyl Arg17) protein at nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. Histone H3R17me2 (dimethyl Arg17) antibody dilution: 1:500.

Application

Histone H3R17me2 (dimethyl Arg17) antibody detects Histone H3R17me2 (dimethyl Arg17) protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Histone H3R17me2 (dimethyl Arg17) antibody dilution: 1:500.

Application

Histone H3R17me2 (dimethyl Arg17) antibody detects Histone H3R17me2 (dimethyl Arg17) protein at nucleus on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Histone H3R17me2 (dimethyl Arg17) antibody dilution: 1:500.

Application

Dotblot analysis of anti-Histone3 (di-methyl Arg17) antibody with peptide samples. Peptide samples (0.1 µg) were spotted onto positively charged nylon membrane and blotted with Histone3 (di-methyl Arg17) antibody at different dilution as indicated. A: Peptide samples of Histone3 (di-methyl Arg17) B: Peptide samples of Histone H3.1

Application

Histone3 (di-methyl Arg17) antibody detects HIST1H3A protein at nucleus on AGS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded AGS xenograft. Histone3 (di-methyl Arg17) antibody dilution: 1:500.

Application

Histone3 (di-methyl Arg17) antibody detects HIST1H3A protein by Western blot analysis. A. 30 µg 293T whole cell lysate/extract B. 30 µg A431 whole cell lysate/extract C. 30 µg HeLa whole cell lysate/extract D. 30 µg HepG2 whole cell lysate/extract 15 % SDS-PAGE Histone3 (di-methyl Arg17) antibody dilution: 1:1000
Positive Control 293T , A431 , HeLa , HepG2
Application Notes *Optimal dilutions/concentrations should be determined by the researcher. :
Additional Information
Form Liquid
Storage Instructions Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
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