Application
Histone H3S10ph (phospho Ser10) antibody [GT921] detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunofluorescent analysis. Samples: HCT116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 protein stained by p21 Cip1 antibody diluted at 1:1000. Red: Histone H3S10ph (phospho Ser10) protein stained by Histone H3S10ph (phospho Ser10) antibody [GT921] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Histone H3 (phospho Ser10) antibody [GT921] detects Histone H3 (phospho Ser10) protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated) | B. 30 µg HeLa whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) 15 % SDS-PAGE Histone H3 (phospho Ser10) antibody [GT921] dilution: 1:1000Application
Histone H3S10ph (phospho Ser10) antibody [GT921] detects Histone H3S10ph (phospho Ser10) protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Histone H3S10ph (phospho Ser10) antibody [GT921] diluted at 1:250.| Product Name | Histone H3S10ph (phospho Ser10) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [GT921] to Histone H3 (phospho Ser10) (histone cluster 1, H3a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Modification | p-Ser10 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GT921 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, ChimpanzeeMouse (100%), Chimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | histone cluster 1 H3a H3/A HIST1H3A H3FA |
|---|---|
| Target | Histone H3 (phospho Ser10) |
| Cellular Localization | Nucleus |
Application
Histone H3S10ph (phospho Ser10) antibody [GT921] detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunofluorescent analysis. Samples: HCT116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: p21 Cip1 protein stained by p21 Cip1 antibody diluted at 1:1000. Red: Histone H3S10ph (phospho Ser10) protein stained by Histone H3S10ph (phospho Ser10) antibody [GT921] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Histone H3 (phospho Ser10) antibody [GT921] detects Histone H3 (phospho Ser10) protein by western blot analysis. A. 30 µg HeLa whole cell lysate/extract (untreated) | B. 30 µg HeLa whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr) 15 % SDS-PAGE Histone H3 (phospho Ser10) antibody [GT921] dilution: 1:1000
Application
Histone H3S10ph (phospho Ser10) antibody [GT921] detects Histone H3S10ph (phospho Ser10) protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Histone H3S10ph (phospho Ser10) antibody [GT921] diluted at 1:250.| Positive Control | Nocodazole treatmented HeLa cell (24hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
