Application
IHC-P analysis of human malignant lymphoma tissue using Histone H3S28ph (phospho Ser28) antibody in the absence (left) and the presence (right) of blocking peptide.Application
WB analysis of extracts from serum-treated HuvEc cells using Histone H3S28ph (phospho-Ser28) antibody (left). Additional analysis with blocking peptide pre-incubated Histone H3S28ph (phospho Ser28) antibody was also perform (right).| Product Name | Histone H3S28ph (phospho Ser28) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H3S28ph (phospho Ser28)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] |
| Modification | p-Ser28 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500-1:1000,IHC: 1:50-1:100,ELISA: 1:4000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H3/i H3FI |
|---|---|
| Target | Histone H3.1 (phospho S28) |
Application
IHC-P analysis of human malignant lymphoma tissue using Histone H3S28ph (phospho Ser28) antibody in the absence (left) and the presence (right) of blocking peptide.
Application
WB analysis of extracts from serum-treated HuvEc cells using Histone H3S28ph (phospho-Ser28) antibody (left). Additional analysis with blocking peptide pre-incubated Histone H3S28ph (phospho Ser28) antibody was also perform (right).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500-1:1000,IHC: 1:50-1:100,ELISA: 1:4000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS (without Mg2+and Ca2+), pH 7.4, 150mM NaCl,0.02% sodium azide and 50% glycerol. |
