| Product Name | Histone H3T3ph (phospho Thr3) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP1702Y] to Histone H3 (phospho Thr3)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. [provided by RefSeq, Jul 2008] |
| Immunogen | A phospho specific peptide corresponding to residues surrounding Threonine 3 of human Histone H3. |
| Modification | p-Thr3 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP1702Y |
| Host Species | Rabbit |
| Tested Applications | FACSICC/IFIHCIHC-PWB |
| FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | H3F3 H3F3A H3 histone family 3A H3.3A |
|---|---|
| Target | Histone H3 (phospho Thr3) |
| Tissue Specificity | This antibody detects Histone H3 phosphorylated at Theronine 3. Species cross reactivity was determined by western blot only. No signal was detected in the following Mouse and Rat tissues lysates: brain, heart, kidney, and spleen. |
| Application Notes | FACS:1:200~1:500 ICC/IF:1:200~1:500 IHC:1:50~1:200 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. |
| Storage Buffer | PBS containing 50% Glycerol, 0.05% BSA and 0.01% Sodium azide. |
