Application
RM208 specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) at higher concentrations. No crossreactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.Application
Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM208 at 1 ug/mL,showed a band of Histone H4 trimethylated at Lysine20 in HeLa cells.| Product Name | Histone H4 (Tri-Methyl Lys20) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Monoclonal antibody [RM208] to Histone H4 (Tri-Methyl Lys20) |
| Immunogen | A trimethyl-peptide corresponding to trimethyl-Histone H4 (Lys20) |
| Modification | Tri-Methyl Lys20 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | RM208 |
| Host Species | Rabbit |
| Tested Applications | ELISAWBMultiplexing |
| Western Blot: 1 ug/mL – 2 ug/mL, ELISA: 0.5 ug/mL - 1 ug/mL, Multiplex: 0.1 ug/mL – 0.5 ug/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Histone H4 (Tri-Methyl Lys20) |
|---|---|
| Tissue Specificity | This antibody reacts to Histone H4 trimethylated at Lysine 20 (K20me3). It may slightly cross react to dimethylated Lysine 20 (K20me2) at a higher concentration. No cross reactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4. |
Application
RM208 specifically reacts to Histone H4 trimethylated at Lysine 20 (K20me3). May slightly cross react to dimethylated Lysine 20 (K20me2) at higher concentrations. No crossreactivity with non-modified Lysine 20, monomethylated Lysine 20 (K20me1), or other methylations in histone H4.
Application
Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using RM208 at 1 ug/mL,showed a band of Histone H4 trimethylated at Lysine20 in HeLa cells.| Application Notes | Western Blot: 1 ug/mL – 2 ug/mL, ELISA: 0.5 ug/mL - 1 ug/mL, Multiplex: 0.1 ug/mL – 0.5 ug/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20.0°C |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
