Application
Western blot analysis of Histone H4 expression in TSA treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Product Name | Histone H4 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H4Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthesized peptide derived from human Histone H4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H4FA histone cluster 1 H4a HIST1H4A |
|---|---|
| Target | Histone H4 |
| Tissue Specificity | HIST1H4A |
Application
Western blot analysis of Histone H4 expression in TSA treated COS7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Application Notes | WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
