Application
ChIP was performed with HeLa nuclear extract and either 1 μg of H4pan antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to Myoglobin or β-Actin.Application
Dot blot analysis of peptide used for immunization and other peptides containing unmodified sequences of different histones using Histone H4 antibody at a dilution of 1:10,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.Application
ELISA was performed using a serial dilution of Histone H4 antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:11,250.| Product Name | Histone H4 antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H4 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAChIP |
| ChIP (2-5 μg/IP), ELISA (1:500), Dot Blotting (1:10.000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H4FA histone cluster 1 H4a HIST1H4A |
|---|---|
| Target | Histone H4 |
Application
ChIP was performed with HeLa nuclear extract and either 1 μg of H4pan antibody or control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to Myoglobin or β-Actin.
Application
Dot blot analysis of peptide used for immunization and other peptides containing unmodified sequences of different histones using Histone H4 antibody at a dilution of 1:10,000. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane for analysis.
Application
ELISA was performed using a serial dilution of Histone H4 antibody in antigen coated wells. By plotting the absorbance against the antibody dilution, the titer of the antibody was estimated to be 1:11,250.| Application Notes | ChIP (2-5 μg/IP), ELISA (1:500), Dot Blotting (1:10.000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.05% azide and 0.05% ProClin 300. |
