Application
Histone H4 (mono methyl Lys20) antibody detects Histone H4 (mono methyl Lys20) protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (mono methyl Lys20) antibody dilution: 1:1000Application
Dotblot analysis of anti-Histone H4 (mono methyl Lys20) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (mono methyl Lys20) antibody 1:3000 dilution. A: Peptide samples of H4K20 un B: Peptide samples of H4K20me1 C: Peptide samples of H4K20me2 D: Peptide samples of H4K20me3Application
Histone H4 (mono methyl Lys20) antibody detects Histone H4 (mono methyl Lys20) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (mono methyl Lys20) protein stained by Histone H4 (mono methyl Lys20) antibody diluted at 1:500. Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Histone H4K20me1 (monomethyl Lys20) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H4 (mono methyl Lys20) (histone cluster 1, H4a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq] |
| Modification | monomethyl Lys20 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Xenopus laevisMouse (100%), Xenopus laevis (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H4FA histone cluster 1 H4a HIST1H4A |
|---|---|
| Target | Histone H4 (mono methyl Lys20) |
| Cellular Localization | Nucleus |
Application
Histone H4 (mono methyl Lys20) antibody detects Histone H4 (mono methyl Lys20) protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract B. 30 µg HeLa nuclear lysate/extract 15 % SDS-PAGE Histone H4 (mono methyl Lys20) antibody dilution: 1:1000
Application
Dotblot analysis of anti-Histone H4 (mono methyl Lys20) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (mono methyl Lys20) antibody 1:3000 dilution. A: Peptide samples of H4K20 un B: Peptide samples of H4K20me1 C: Peptide samples of H4K20me2 D: Peptide samples of H4K20me3
Application
Histone H4 (mono methyl Lys20) antibody detects Histone H4 (mono methyl Lys20) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (mono methyl Lys20) protein stained by Histone H4 (mono methyl Lys20) antibody diluted at 1:500. Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | HeLa , HeLa nuclear |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
