Application
Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Histone H4 (Acetyl-Lys5) Antibody. The lane on the right is treated with the synthesized peptide.Application
Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide.Application
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H4 (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | Histone H4K5ac (acetyl Lys5) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H4 (Acetyl Lys5)Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Histone H4 around the acetylated site of Lys5. |
| Modification | acetyl Lys5 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHCIHC-PWB |
| IHC: 1:50~1:100 : |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | H4F2 HIST HIST1H4A Histone H4 Histone H4 (acetyl K5) Histone H4 (acetyl Lys5) Histone H4 acetyl K5 Histone H4 acetyl Lys5 Acetyl Histone H4 (K5) Acetyl Histone H4 (Lys5) |
|---|---|
| Target | Histone H4 |
| Molecular Weight(MW) | 11 kDa (note) |
| Tissue Specificity | Histone H4 (Acetyl-Lys5) Antibody detects endogenous levels of total Histone H4 protein only when acetylated at lysine5. |
Application
Western blot analysis of extracts from COS7 cells, treated with TSA 400nM 24h, using Histone H4 (Acetyl-Lys5) Antibody. The lane on the right is treated with the synthesized peptide.
Application
Immunofluorescence analysis of HeLa cells, using Histone H4 (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide.
Application
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Histone H4 (Acetyl-Lys5) Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | IHC: 1:50~1:100 : |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
