Application
Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-Histone H4K8ac (acetyl Lys8) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.Application
Histone H4 (acetyl K9) antibody immunoprecipitates Histone H4 (acetyl K9) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H4 (acetyl K9) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 μg of Histone H4 (acetyl K9) antibody . 12% SDS-PAGE The immunoprecipitated Histone H4 (acetyl K9) protein was detected by Histone H4 (acetyl K9) antibody diluted at 1 : 5000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.Application
Histone H4 (acetyl Lys8) antibody detects Histone H4 (acetyl Lys8) protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 µg HeLa whole cell lysate/extract (0.4 µM Trichostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H4 (acetyl Lys8) antibody dilution: 1:5000Application
Histone H4K8ac (acetyl Lys8) antibody detects Histone H4K8ac (acetyl Lys8) protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.Application
Histone H4K8ac (acetyl Lys8) antibody detects Histone H4K8ac (acetyl Lys8) protein at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.Application
Histone H4K8ac (acetyl Lys8) antibody detects Histone H4K8ac (acetyl Lys8) protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.Application
Dotblot analysis of anti-Histone H4 (acetyl Lys9) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys9) antibody 1:5000 dilution. A: Peptide samples of H4K8 un B: Peptide samples of H4K8ac C: Peptide samples of H4K16un D: Peptide samples of H4K16acApplication
Histone H4 (acetyl Lys8) antibody detects Histone H4 (acetyl Lys8) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (acetyl Lys8) protein stained by Histone H4 (acetyl Lys8) antibody diluted at 1:500. Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Histone H4K8ac (acetyl Lys8) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Histone H4 (acetyl Lys8) (histone cluster 1, H4a)Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq] |
| Modification | acetyl Lys8 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DotICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevisXenopus laevis (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | H4FA histone cluster 1 H4a HIST1H4A |
|---|---|
| Target | Histone H4 (acetyl Lys8) |
| Cellular Localization | Nucleus |
Application
Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-Histone H4K8ac (acetyl Lys8) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.
Application
Histone H4 (acetyl K9) antibody immunoprecipitates Histone H4 (acetyl K9) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H4 (acetyl K9) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 μg of Histone H4 (acetyl K9) antibody . 12% SDS-PAGE The immunoprecipitated Histone H4 (acetyl K9) protein was detected by Histone H4 (acetyl K9) antibody diluted at 1 : 5000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.
Application
Histone H4 (acetyl Lys8) antibody detects Histone H4 (acetyl Lys8) protein by Western blot analysis. A. 30 µg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr) B. 30 µg HeLa whole cell lysate/extract (0.4 µM Trichostatin A treatment for 18 hr) 15 % SDS-PAGE Histone H4 (acetyl Lys8) antibody dilution: 1:5000
Application
Histone H4K8ac (acetyl Lys8) antibody detects Histone H4K8ac (acetyl Lys8) protein at nucleus on mouse duodenum by immunohistochemical analysis. Sample: Paraffin-embedded mouse duodenum. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.
Application
Histone H4K8ac (acetyl Lys8) antibody detects Histone H4K8ac (acetyl Lys8) protein at nucleus on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.
Application
Histone H4K8ac (acetyl Lys8) antibody detects Histone H4K8ac (acetyl Lys8) protein at nucleus on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. Histone H4K8ac (acetyl Lys8) antibody dilution: 1:500.
Application
Dotblot analysis of anti-Histone H4 (acetyl Lys9) antibody with peptide samples. Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys9) antibody 1:5000 dilution. A: Peptide samples of H4K8 un B: Peptide samples of H4K8ac C: Peptide samples of H4K16un D: Peptide samples of H4K16ac
Application
Histone H4 (acetyl Lys8) antibody detects Histone H4 (acetyl Lys8) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Histone H4 (acetyl Lys8) protein stained by Histone H4 (acetyl Lys8) antibody diluted at 1:500. Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
