Application
ChIP was performed with U2OS nuclear extract and either 15 μl of H4K8ac antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to ALDOA promoter or MyoD.Application
Dot blot analysis of peptides containing modified histone H4 and H3 and an unmodified histone H4 sequence uaing H4K8ac antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane for analysis.Application
WB analysis of histone extracts (15 μg) from HeLa cells using H4K8ac antibody diluted 1:250 in TBS-Tween containing 5% skimmed milk. Lane 2 shows the result of the WB analysis with the antibody; lane 1 shows the same analysis after incubation of the antibody with 750 pmol blocking peptide for 1 hour at room temperature.Application
ELISA was performed using a serial dilution of Histone H4K8ac (acetyl Lys8) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:17,500.| Product Name | Histone H4K8ac (acetyl Lys8) antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Histone H4 (acetyl Lys8) |
| Modification | acetyl Lys8 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | DotELISAWBChIP |
| ChIP (5 µl/ChIP), ELISA (1:500), Dot blotting (1:20,000), Western blotting (1:250): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Histone H4 acetyl Lys8 H4FA acetyl Histone H4 histone cluster 1 H4a HIST1H4A acetyl Lys8 Histone H4 Histone H4 acetyl acetyl K8 Histone H4 Histone H4 acetyl K8 |
|---|---|
| Target | Histone H4 (acetyl Lys8) |
Application
ChIP was performed with U2OS nuclear extract and either 15 μl of H4K8ac antibody or 5 μg of control rabbit IgG. The precipitated DNA was detected by QPCR with primer set targeting to ALDOA promoter or MyoD.
Application
Dot blot analysis of peptides containing modified histone H4 and H3 and an unmodified histone H4 sequence uaing H4K8ac antibody at a dilution of 1:20,000. One hundred to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane for analysis.
Application
WB analysis of histone extracts (15 μg) from HeLa cells using H4K8ac antibody diluted 1:250 in TBS-Tween containing 5% skimmed milk. Lane 2 shows the result of the WB analysis with the antibody; lane 1 shows the same analysis after incubation of the antibody with 750 pmol blocking peptide for 1 hour at room temperature.
Application
ELISA was performed using a serial dilution of Histone H4K8ac (acetyl Lys8) antibody in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 2), the titer of the antibody was estimated to be 1:17,500.| Application Notes | ChIP (5 µl/ChIP), ELISA (1:500), Dot blotting (1:20,000), Western blotting (1:250): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Whole antiserum from rabbit containing 0.05% azide. |
