Application
HLA DQB1 antibody detects HLA DQB1 protein at cell membrane and endoplasmic reticulum by immunofluorescent analysis. Sample: Raji cells were fixed in iced-cold MeOH for 5 min. Green: HLA DQB1 protein stained by HLA DQB1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
HLA DQB1 antibody detects HLA DQB1 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract 12% SDS-PAGE HLA DQB1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HLA DQB1 antibody detects HLA DQB1 protein at cytoplasm and membrane on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. HLA DQB1 antibody diluted at 1:500.| Product Name | HLA DQB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HLA DQB1 (major histocompatibility complex, class II, DQ beta 1)HLA-DQB1 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and it contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HLA DQB1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HLA-DQB1 HLA-DQB IDDM1 major histocompatibility complex class II DQ beta 1 CELIAC1 |
|---|---|
| Target | HLA DQB1 |
Application
HLA DQB1 antibody detects HLA DQB1 protein at cell membrane and endoplasmic reticulum by immunofluorescent analysis. Sample: Raji cells were fixed in iced-cold MeOH for 5 min. Green: HLA DQB1 protein stained by HLA DQB1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
HLA DQB1 antibody detects HLA DQB1 protein by western blot analysis. A. 30 µg Jurkat whole cell lysate/extract 12% SDS-PAGE HLA DQB1 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HLA DQB1 antibody detects HLA DQB1 protein at cytoplasm and membrane on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. HLA DQB1 antibody diluted at 1:500.| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
