Application
FACS analysis of human peripheral blood lymphocytes using HLA-A2 antibody [BB7.2].| Product Name | HLA-A2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [BB7.2] to HLA-A2HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | Papain solubilised HLA-A2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | BB7.2 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIP |
| Flow Cytometry: Max Dilution 10 ug/ml Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | major histocompatibility complex class I A HLA-A HLAA |
|---|---|
| Target | HLA-A2 |
| Tissue Specificity | This antibody recognizes the human HLA-A2 histocompatability antigen. |
Application
FACS analysis of human peripheral blood lymphocytes using HLA-A2 antibody [BB7.2].| Application Notes | Flow Cytometry: Max Dilution 10 ug/ml Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide |
