Application
WB analysis of extracts from various cell lines using HLA-A antibody.Application
IHC-P analysis of human endometrial cancer using HLA-A antibody at a dilution of 1:500.Application
IHC-P analysis of human neurilemmoma using HLA-A antibody at a dilution of 1:500.Application
IHC-P analysis of mouse kidney tissue using HLA-A antibody at a dilution of 1:500.Application
IHC-P analysis of mouse liver tissue using HLA-A antibody at a dilution of 1:500.Application
ICC/IF analysis of U2OS cells using HLA-A antibody (green) and DAPI (blue).| Product Name | HLA-A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HLA-AHLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human HLA-A |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:200. IHC: 1:50 - 1:20. IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HLAA major histocompatibility complex class I A |
|---|---|
| Target | HLA-A |
| Molecular Weight(MW) | 41 kDa (note) |
Application
WB analysis of extracts from various cell lines using HLA-A antibody.
Application
IHC-P analysis of human endometrial cancer using HLA-A antibody at a dilution of 1:500.
Application
IHC-P analysis of human neurilemmoma using HLA-A antibody at a dilution of 1:500.
Application
IHC-P analysis of mouse kidney tissue using HLA-A antibody at a dilution of 1:500.
Application
IHC-P analysis of mouse liver tissue using HLA-A antibody at a dilution of 1:500.
Application
ICC/IF analysis of U2OS cells using HLA-A antibody (green) and DAPI (blue).| Application Notes | WB: 1:500 - 1:200. IHC: 1:50 - 1:20. IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
