Application
Staining of peripheral blood lymphocytes using HLA-ABC antibody .| Product Name | HLA-A, HLA-B + HLA-C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [W6/32.] to HLA Class IHLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may loose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC. |
| Immunogen | The cell membranes of human tonsil lymphocytes |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | W6/32 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSICC/IFIHC-F |
| For IHC(frozen) a three-step avidin-biotin complex system, a dilution of 1:50 to 1:100 may be used as a guideline. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good dilutent to use with most antibodies. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HLA-F HLA-A HLAC HLA-C HLAA HLAF HLAG major histocompatibility complex class I HLAA HLAB + HLAC HLA-G FLJ26655 AS D6S204 HLA-B27 FLJ27082 HLA-B73 HLA-Cw HLAB HLA-JY3 MGC111087 HLC-C SPDA1 PSORS1 HLA F HLA A HLA C HLA G HLC C H |
|---|---|
| Target | HLA-A, HLA-B + HLA-C |
| Tissue Specificity | This antibody reacts with a monomorphic epitope of the 45 kD polypeptide, constiituting the HLAA,-B and-C or class I antigen of the human major histocompatibility complex. |
| Cellular Localization | Cell membrane |
| Light Chain Type | kappa |
Application
Staining of peripheral blood lymphocytes using HLA-ABC antibody .| Application Notes | For IHC(frozen) a three-step avidin-biotin complex system, a dilution of 1:50 to 1:100 may be used as a guideline. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good dilutent to use with most antibodies. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 0.1% sodium azide |
