Application
Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using HLA-B antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: HL-60 10% SDS PAGE diluted at 1:1000| Product Name | HLA-B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HLA-B (major histocompatibility complex, class I, B)HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HLA-B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AS antibody HLA-B27 antibody HLA-B73 antibody HLAB antibody MGC111087 antibody SPDA1 antibody HLA-B antibody MHC class I antigen SHCHA antibody MHC HLA-B transmembrane glycoprotein antibody lymphocyte antigen antibody MHC Class I HLA heavy chain |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) (2) |
| Tissue Specificity | This antibody may cross with other MHC class I members. |
| Cellular Localization | Membrane |
Application
Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using HLA-B antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: HL-60 10% SDS PAGE diluted at 1:1000| Positive Control | HL-60 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
