Rabbit Polyclonal antibody to HLA-DPA1HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules. [provided by RefSeq, Jul 2008]
Immunogen
A synthetic peptide corresponding to an Internal region of Human HLA-DPA1
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Predicted Reactivity
Human: 100%; Pig: 86%
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
HLA DP1A DP(W4) PLT1 DP(W3) HLA-DPA1 HLADP HLASB
Target
HLA-DPA1
Molecular Weight(MW)
29 KDa (note)
Application
Application
WB analysis of human fetal thymus tissue using HLA-DPA1 antibody at 0.2-1μg/ml.
Positive Control
human fetal thymus
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.