Application
HLA-DR antibody [N2C3] detects HLA-DR protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. HLA-DR antibody [N2C3] diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE diluted at 1:1000| Product Name | HLA-DR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HLA-DRB1 (major histocompatibility complex, class II, DR beta 1)HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HLA-DR. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | DogDog (86%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DRB1 antibody DRw10 antibody FLJ75017 antibody FLJ76359 antibody HLA-DR1B antibody HLA-DRB antibody HLA-DRB1* antibody SS1 antibody HLA-DRB1 antibody lymphocyte antigen DRB1 antibody human leucocyte antigen DRB1 antibody MHC class II antigen ant |
|---|---|
| Target | HLA-DRB1 |
| Molecular Weight(MW) | 30 kDa (note) (3) |
| Cellular Localization | Membrane |
Application
HLA-DR antibody [N2C3] detects HLA-DR protein at cytoplasm in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. HLA-DR antibody [N2C3] diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: H1299 12% SDS PAGE diluted at 1:1000| Positive Control | A431 , H1299 , HeLa , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
