Application
The antibody stains infiltrating macrophages in pulmonary diseases. In the top left corner see detail of macropage.| Product Name | HLA-G antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Antibody to Human HLA - GHLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells. |
| Immunogen | Denaturated heavy chain full-length recombinant human HLA-G |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-G/1 |
| Host Species | Mouse |
| Tested Applications | IHCWB |
| For IHC-P: Use at an assay dependent dilution. This product requires heat mediated antigen retrieval (sodium citrate buffer pH 6.0) prior to staining of paraffin sections. For WB: Use at 1:250-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 142871 HLAG P17693 MHC-G MHCG 3135 HLA-G HLA G MHC G |
|---|---|
| Target | HLA-G |
| Tissue Specificity | Recognises denatured free HLA-G but does not recognise HLA-G bound to the cell surface. |
Application
The antibody stains infiltrating macrophages in pulmonary diseases. In the top left corner see detail of macropage.| Positive Control | Placenta (human) |
|---|---|
| Application Notes | For IHC-P: Use at an assay dependent dilution. This product requires heat mediated antigen retrieval (sodium citrate buffer pH 6.0) prior to staining of paraffin sections. For WB: Use at 1:250-1:1,000. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 0.09% sodium azide |
