Application
Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.Application
Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) and protein G. HLA-G was detected by anti-human HLA-G and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).| Product Name | HLA-G antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Monoclonal Antibody to HLA-G (Human) Azide freeHLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq] |
| Immunogen | Recombinant human HLA-G refolded with beta2-microglobulin and peptide. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MEM-G/9 |
| Host Species | Mouse |
| Tested Applications | ELISAFACSIHC-FIPWB |
| FACS: Recommended dilution: 2-5 ug/ml. Positive control: JEG-3 human choriocarcinoma cell line.ELISA: Coating antibody (10 ug/ml) Detection antibody (biotin or peroxidase conjugate (1 ug/ml): |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 142871 HLAG P17693 MHC-G MHCG 3135 HLA-G HLA G MHC G |
|---|---|
| Target | HLA-G |
| Tissue Specificity | The antibody reacts with native form of human HLA-G1 on the cell surface as well as with soluble HLA-G5 isoform in its beta2-microglobulin associated form. The antibody MEM-G/9 is standard reagent thoroughly validated during 3rd International Conference on HLA-G (Paris, 2003). |
Application
Double surface staining of HLA-G1 transfectants (viable cells gate) using anti-human HLA-G (MEM-G/9) APC and anti-human HLA-G (MEM-G/11) FITC.
Application
Immunoprecipitation of HLA-G from HLA-G1 transfectants (LCL-HLA-G1) by anti-human HLA-G (MEM-G/9) and protein G. HLA-G was detected by anti-human HLA-G and goat anti-mouse HRP in cell lysate (Lane 1) and in the immunoprecipitate (Lane 2).| Application Notes | FACS: Recommended dilution: 2-5 ug/ml. Positive control: JEG-3 human choriocarcinoma cell line.ELISA: Coating antibody (10 ug/ml) Detection antibody (biotin or peroxidase conjugate (1 ug/ml): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4 |
