Application
HLTF?antibody immunoprecipitates HLTF?protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HLTF?protein by 2 μg of HLTF?antibody 7.5% SDS-PAGE The immunoprecipitated HLTF?protein was detected by HLTF?antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HLTF antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:5000Application
Immunohistochemical analysis of paraffin-embedded MB157 xenograft, using HLTF antibody at 1:500 dilution.| Product Name | HLTF antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HLTF (helicase-like transcription factor)This gene encodes a member of the SWI/SNF family. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein contains a RING finger DNA binding motif. Two transcript variants encoding the same protein have been found for this gene. However, use of an alternative translation start site produces an isoform that is truncated at the N-terminus compared to the full-length protein. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HLTF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (81%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HIP116 antibody HIP116A antibody HLTF1 antibody RNF80 antibody SMARCA3 antibody SNF2L3 antibody ZBU1 antibody HLTF antibody SNF2-like 3 antibody SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily a member 3 anti |
|---|---|
| Molecular Weight(MW) | 114 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
HLTF?antibody immunoprecipitates HLTF?protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of HLTF?protein by 2 μg of HLTF?antibody 7.5% SDS-PAGE The immunoprecipitated HLTF?protein was detected by HLTF?antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HLTF antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 7.5% SDS PAGE diluted at 1:5000
Application
Immunohistochemical analysis of paraffin-embedded MB157 xenograft, using HLTF antibody at 1:500 dilution.| Positive Control | HeLa |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
