Application
HMBOX1 antibody [N3C3] detects HMBOX1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMBOX1 protein stained by HMBOX1 antibody [N3C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Homeobox-containing protein 1 antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | HMBOX1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HMBOX1 (homeobox containing 1)Transcription factor. Isoform 1 acts as a transcriptional repressor. Isoform 4 has very low activity as a transcriptional repressor. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HMBOX1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Xenopus laevis, Chicken, BovineMouse (98%), Xenopus laevis (97%), Chicken (97%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ21616 antibody HNF1LA antibody PBHNF antibody HMBOX1 antibody homeobox-containing protein PBHNF antibody homeobox-containing protein 1 antibody homeobox containing 1 antibody |
|---|---|
| Target | Homeobox-containing protein 1 |
| Molecular Weight(MW) | 47 kDa (note) (6) |
| Cellular Localization | Nucleus |
Application
HMBOX1 antibody [N3C3] detects HMBOX1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMBOX1 protein stained by HMBOX1 antibody [N3C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Homeobox-containing protein 1 antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
