Application
Immunofluorescence analysis of HepG2 cells, using HMG14 (Ab-21) Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | HMG14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HMG14Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation. Inhibits the phosphorylation of nucleosomal histones H3 and H2A by RPS6KA5/MSK1 and RPS6KA3/RSK2. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human HMG14. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IF |
| ELISA: 1:1000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Nonhistone chromosomal protein HMG-14 Highmobility group nucleosome binding domain 1 Nonhistone chromosomal protein HMG14 High-mobility group nucleosome binding domain 1 Highmobility group nucleosome binding domain1 HMG14 |
|---|---|
| Target | HMG14 |
| Molecular Weight(MW) | 10 kDa (note) |
| Tissue Specificity | HMG14 (Ab-21) Antibody detects endogenous levels of total HMG14 protein. |
| Cellular Localization | Cytoplasmic enrichment upon phosphorylation. The RNA edited version localizes to the nucleus. |
Application
Immunofluorescence analysis of HepG2 cells, using HMG14 (Ab-21) Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:1000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
