Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody [N1N3] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.Application
HMGA1 antibody [N1N3] detects HMGA1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody [N1N3] diluted at 1:500.| Product Name | HMGA1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HMGA1 (high mobility group AT-hook 1)This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGA1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Rhesus Monkey, ChimpanzeeMouse (100%), Rat (100%), Dog (100%), Pig (100%), Rhesus Monkey (100%), Chimpanzee (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HMG-R antibody HMGA1A antibody HMGIY antibody MGC12816 antibody MGC4242 antibody MGC4854 antibody HMGA1 antibody high mobility group protein HMG-I/HMG-Y antibody high-mobility group (nonhistone chromosomal) protein isoforms I and Y antibody high m |
|---|---|
| Target | HMGA1 |
| Molecular Weight(MW) | 12 kDa (note) (12) |
| Cellular Localization | Nucleus |
Application
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody [N1N3] diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
Application
HMGA1 antibody [N1N3] detects HMGA1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA1 antibody [N1N3] diluted at 1:500.| Positive Control | 293T , H1299 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
