Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HMGA2 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using HMGA2 antibody at 1:500 dilution.Application
HMGA2 antibody - ChIP grade detects HMGA2 protein at nucleus in human HuCCT1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human HuCCT1 xenograft . HMGA2 antibody - ChIP grade diluted at 1:250.Application
HMGA2 antibody detects HMGA2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HMGA2 antibody detects HMGA2 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HMGA2 antibody detects HMGA2 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HMGA2 antibody diluted at 1:500.Application
HMGA2 antibody detects HMGA2 protein at nucleus in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. HMGA2 antibody diluted at 1:500.Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HMGA2 antibody detects HMGA2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGA2 protein stained by HMGA2 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.Application
Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody . Western blot analysis was performed using HMGA2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | HMGA2 antibody - ChIP grade |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HMGA2 (high mobility group AT-hook 2)This gene encodes a protein that belongs to the non-histone chromosomal high mobility group (HMG) protein family. HMG proteins function as architectural factors and are essential components of the enhancesome. This protein contains structural DNA-binding domains and may act as a transcriptional regulating factor. Identification of the deletion, amplification, and rearrangement of this gene that are associated with myxoid liposarcoma suggests a role in adipogenesis and mesenchymal differentiation. A gene knock out study of the mouse counterpart demonstrated that this gene is involved in diet-induced obesity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HMGA2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Zebrafish, ChickenZebrafish (80%), Chicken (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BABL antibody HMGI-C antibody HMGIC antibody LIPO antibody STQTL9 antibody HMGA2 antibody high mobility group AT-hook protein 2 antibody High-mobility group protein HMGI-C antibody high-mobility group (nonhistone chromosomal) protein isoform I-C an |
|---|---|
| Molecular Weight(MW) | 12 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Immunohistochemical analysis of paraffin-embedded H1299 xenograft, using HMGA2 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using HMGA2 antibody at 1:500 dilution.
Application
HMGA2 antibody - ChIP grade detects HMGA2 protein at nucleus in human HuCCT1 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded human HuCCT1 xenograft . HMGA2 antibody - ChIP grade diluted at 1:250.
Application
HMGA2 antibody detects HMGA2 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody diluted at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HMGA2 antibody detects HMGA2 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HMGA2 antibody detects HMGA2 protein at nucleus in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. HMGA2 antibody diluted at 1:500.
Application
HMGA2 antibody detects HMGA2 protein at nucleus in rat duodenum by immunohistochemical analysis. Sample: Paraffin-embedded rat duodenum. HMGA2 antibody diluted at 1:500.
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with HMGA2 antibody - ChIP grade diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HMGA2 antibody detects HMGA2 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HMGA2 protein stained by HMGA2 antibody diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.
Application
Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody . Western blot analysis was performed using HMGA2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | A431 , HeLa , HepG2 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
