| Product Name | HMGB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [KS1] to HMGB1HMG1 and HMG2, chromatin non-histone high mobility group proteins 1 and 2, are 27 and 25 kDa members of a family of proteins containing multiple HMG-boxes, conserved domains of 80 amino acids which mediate DNA binding of many proteins. HMG box domains recognize DNA structure, such as prebent, supercoiled or four way junction DNA, and non- specific DNA sequences. Both HMG1 and HMG2 contain an N-terminal HMG box, a central HMG box, and an acidic carboxy terminus. The acidic tails of these proteins contain multiple serine residues which match the phosphorylation consensus sites of casein kinase II, and phosphorylation of this domain appears to be important for proper functioning of these proteins. HMG1/2 have been shown to facilitate the binding of various sequence-specific transcription factors to their respective DNA binding sites, such as steroid hormone receptors, p53, and Oct. HMG1/2 may serve as architectural factors that recognize and mediate DNA structural changes that accompany various events such as DNA repair, transcription and replication. |
| Immunogen | Pig HMGB1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | KS1 |
| Host Species | Mouse |
| Tested Applications | WB |
| For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | Amphoterin High mobility group1 DKFZp686A04236 High mobility group protein B1 HMG3 High mobility group protein 1 HMGB1 SBP-1 Nonhistone chromosomal protein HMG1 Sulfoglucuronyl carbohydrate binding protein Hmgb1 protein High mobility group 1 HM |
|---|---|
| Target | HMGB1 |
| Positive Control | HeLa Cell Lysate |
|---|---|
| Application Notes | For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-bufferd saline |
