Application
HNF1 alpha antibody [GT4110] detects HNF1 alpha protein at nucleus in human SW480 by immunohistochemical analysis. Sample: Paraffin-embedded human SW480. HNF1 alpha antibody [GT4110] diluted at 1:250.Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [GT4110] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.| Product Name | HNF1 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody to HNF1A (HNF1 homeobox A)The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HNF1 alpha. The exact sequence is proprietary. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | GT4110 |
| Host Species | Mouse |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, Chicken, BovineRat (99%), Xenopus laevis (85%), Pig (99%), Chicken (95%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HNF-1A antibody HNF1 antibody IDDM20 antibody LFB1 antibody MODY3 antibody TCF-1 antibody TCF1 antibody HNF1A antibody albumin proximal factor antibody HNF-1-alpha antibody "transcription factor 1 hepatic antibody" hepatocyte nuclear factor 1-a |
|---|---|
| Molecular Weight(MW) | 67 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
HNF1 alpha antibody [GT4110] detects HNF1 alpha protein at nucleus in human SW480 by immunohistochemical analysis. Sample: Paraffin-embedded human SW480. HNF1 alpha antibody [GT4110] diluted at 1:250.
Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with HNF1 alpha antibody [GT4110] diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.| Positive Control | HeLa , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS |
