Application
ICC/IF analysis of A549 cells using HNF4 alpha antibody (green) and DAPI (blue).Application
WB analysis of extracts from various cell lines using HNF4 alpha antibody.| Product Name | HNF4 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HNF4 alphaThe protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012] |
| Immunogen | The immunogen corresponding to a region within amino acids 241 and 255 of human HNF4 alpha (Uniprot: P41235) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB: 1:500 - 1:200. IHC: 1:50 - 1:20. IF: 1:50 - 1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HNF4a8 NR2A21 HNF4a9 TCF14 HNF4A NR2A1 HNF4 hepatocyte nuclear factor 4 alpha HNF4a7 MODY1 HNF4alpha TCF MODY |
|---|---|
| Target | HNF4 alpha |
| Molecular Weight(MW) | 52 kDa (note) |
Application
ICC/IF analysis of A549 cells using HNF4 alpha antibody (green) and DAPI (blue).
Application
WB analysis of extracts from various cell lines using HNF4 alpha antibody.| Application Notes | WB: 1:500 - 1:200. IHC: 1:50 - 1:20. IF: 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
