Application
HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 10 μg/mL Monoclonal Anti-hnRNP-A1 Clone: 9H10 (Cat. No. ). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.Application
Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-hnRNP-A1 Clone: 9H10 (Cat. No. ). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. COS7 5. MDBK 6. NRK 7. CHOApplication
Application| Product Name | hnRNP A1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [9H10] to hnRNP A1The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It is one of the most abundant core proteins of hnRNP complexes and it is localized to the nucleoplasm. This protein, along with other hnRNP proteins, is exported from the nucleus, probably bound to mRNA, and is immediately re-imported. Its M9 domain acts as both a nuclear localization and nuclear export signal. The encoded protein is involved in the packaging of pre-mRNA into hnRNP particles, transport of poly A+ mRNA from the nucleus to the cytoplasm, and may modulate splice site selection. It is also thought have a primary role in the formation of specific myometrial protein species in parturition. Multiple alternatively spliced transcript variants have been found for this gene but only two transcripts are fully described. These variant have multiple alternative transcription initiation sites and multiple polyA sites. |
| Immunogen | Full length hnRNPA1 native protein (partially purified) from HeLa cells (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 9H10 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIPWB |
| ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. This antibody does not IP the hnRNP complex. WB: Use at an assay dependent dilution. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | HNRPA1 MGC102835 hnRNP A1 hnRNP-A1 hnRNPA1 |
|---|---|
| Target | HNRNP A1 |
| Cellular Localization | Nuclear |
Application
HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 10 μg/mL Monoclonal Anti-hnRNP-A1 Clone: 9H10 (Cat. No. ). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate.
Application
Cell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Monoclonal Anti-hnRNP-A1 Clone: 9H10 (Cat. No. ). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. HEK-293T 2. HeLa 3. G361 4. COS7 5. MDBK 6. NRK 7. CHO
Application
Application| Application Notes | ELISA: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. This antibody does not IP the hnRNP complex. WB: Use at an assay dependent dilution. Detects a band of approximately 34 kDa (predicted molecular weight: 38 kDa). Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: PBS |
