Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using hnRNP F antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft , using hnRNP F antibody at 1:100 dilution.Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunoprecipitation of hnRNP F protein from 293T whole cell extracts using 5 μg of hnRNP F antibody [N1N3] . Western blot analysis was performed using hnRNP F antibody [N1N3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
hnRNP F antibody [N1N3] detects hnRNP F protein at nucleus on mouse uterus by immunohistochemical analysis. Sample: Paraffin-embedded mouse uterus. hnRNP F antibody [N1N3] diluted at 1:500.| Product Name | hnRNP F antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to hnRNP F (heterogeneous nuclear ribonucleoprotein F)This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human hnRNP F. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, Chimpanzee, BovineRat (98%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HNRPF antibody MGC110997 antibody OK/SW-cl.23 antibody mcs94-1 antibody HNRNPF antibody HnRNP F protein antibody heterogeneous nuclear ribonucleoprotein F antibody nucleolin-like protein mcs94-1 antibody |
|---|---|
| Target | hnRNP F |
| Molecular Weight(MW) | 46 kDa (note) (7) |
| Tissue Specificity | Knockdown/Knockout validation was supported by references (PMID:26991008) |
| Cellular Localization | Nucleus , nucleoplasm |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using hnRNP F antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded A549 Xenograft , using hnRNP F antibody at 1:100 dilution.
Application
Sample (30 µg of whole cell lysate) A: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunoprecipitation of hnRNP F protein from 293T whole cell extracts using 5 μg of hnRNP F antibody [N1N3] . Western blot analysis was performed using hnRNP F antibody [N1N3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
hnRNP F antibody [N1N3] detects hnRNP F protein at nucleus on mouse uterus by immunohistochemical analysis. Sample: Paraffin-embedded mouse uterus. hnRNP F antibody [N1N3] diluted at 1:500.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
