Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homeobox C10 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Homeobox C10 antibody detects Homeobox C10 protein by western blot analysis. A.50 µg mouse muscle lysate/extract 10% SDS-PAGE Homeobox C10 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Homeobox C10 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Homeobox C10 antibody at 1:500 dilution.| Product Name | Homeobox C10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Homeobox C10 (homeobox C10)This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, which are located on different chromosomes and consist of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXC genes located in a cluster on chromosome 12. The protein level is controlled during cell differentiation and proliferation, which may indicate this protein has a role in origin activation. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Homeobox C10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | RatRat (97%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HOX3I antibody MGC5259 antibody HOXC10 antibody homeo box C10 antibody homeobox protein Hox-3I antibody homeobox protein Hox-C10 antibody homeoprotein C10 antibody homeobox C10 antibody |
|---|---|
| Molecular Weight(MW) | 38 kDa (note) (2) |
| Cellular Localization | Nucleus |
Application
Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Homeobox C10 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Homeobox C10 antibody detects Homeobox C10 protein by western blot analysis. A.50 µg mouse muscle lysate/extract 10% SDS-PAGE Homeobox C10 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Homeobox C10 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.
Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using Homeobox C10 antibody at 1:500 dilution.| Positive Control | HeLa , HepG2 , mouse muscle |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
