Application
Homer antibody detects Homer protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Homer protein stained by Homer antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .Application
Immunoprecipitation of Homer protein from IMR32 whole cell extracts using 5 μg of Homer antibody . Western blot analysis was performed using Homer antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Homer antibody detects HOMER1 protein by western blot analysis. A. 50 µg rat brain lysate/extract 10% SDS-PAGE Homer antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HOMER1 antibody at 1:100 dilution.Application
Immunofluorescence analysis of methanol-fixed HeLa, using Homer antibody at 1:500 dilution.Application
Homer antibody detects HOMER1 protein by western blot analysis. A. 30 µg IMR32 whole cell lysate/extract 10% SDS-PAGE Homer antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (20 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:20000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Homer antibody detects Homer protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and blotted with Homer antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Homer1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Homer1 (homer homolog 1 (Drosophila))This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Homer1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PIPWBIB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Xenopus laevis, Pig, Rhesus Monkey, BovineXenopus laevis (85%), Pig (98%), Rhesus Monkey (99%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HOMER antibody HOMER1A antibody HOMER1B antibody HOMER1C antibody SYN47 antibody Ves-1 antibody HOMER1 antibody homer protein homolog 1 antibody homer neuronal immediate early gene 1 antibody homer-1 antibody homer homolog 1 (Drosophila) antibo |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) |
| Cellular Localization | Cytoplasm (By similarity) , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density (By similarity) , synapse (By similarity) |
Application
Homer antibody detects Homer protein expression by immunohistochemical analysis. Sample: Frozen sectioned E13.5 Rat brain. Green: Homer protein stained by Homer antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] diluted at 1:500. Blue: Fluoroshield with DAPI .
Application
Immunoprecipitation of Homer protein from IMR32 whole cell extracts using 5 μg of Homer antibody . Western blot analysis was performed using Homer antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Homer antibody detects HOMER1 protein by western blot analysis. A. 50 µg rat brain lysate/extract 10% SDS-PAGE Homer antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded human ovarian cancer, using HOMER1 antibody at 1:100 dilution.
Application
Immunofluorescence analysis of methanol-fixed HeLa, using Homer antibody at 1:500 dilution.
Application
Homer antibody detects HOMER1 protein by western blot analysis. A. 30 µg IMR32 whole cell lysate/extract 10% SDS-PAGE Homer antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (20 µg of whole cell lysate) A: mouse brain 10% SDS PAGE diluted at 1:20000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Homer antibody detects Homer protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and blotted with Homer antibody diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
