Application
The extracts of mouse brain (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Homer1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.WB
RT-qPCR (A, B) and western blot (C, D) were used to measure DCLRE1B and HOMER1 expressions.| Product Name | Homer1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AT1F3] to Homer1Homer1 (Homer protein homolog 1) belongs to Homer family of proteins that regulate signal transduction through, and the trafficking of, glutamate receptors, as well as maintain and regulate extracellular glutamate levels in corticolimbic brain regions. The Homer1 is enriched at excitatory synapses and binds group 1 metabotropic glutamate receptors (mGluRs). |
| Immunogen | Recombinant human Homer1 (1-354aa) purified from E. coli |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | AT1F3 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot: Use at 1:1000 ~ 2000.Optimal working concentrations should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ |
| Purification | Affinity purified |
| Gene Synonyms | Ves1 Homer SYN47 Ves 1 HOMER1 1 Homer protein homolog1 HOMER1B Homer protein homolog 1 HOMER 1A Homer protein homolog 1 Homer 1 HOMER1C neuronal immediate early gene HOMER 1C HOMER 1B Homer protein homolog1 HOMER1A HOMER1A |
|---|---|
| Target | Homer1 |
| Cellular Localization | Postsynaptic density of neuronal cells. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum. |
| Light Chain Type | kappa |
Application
The extracts of mouse brain (50ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Homer1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
WB
RT-qPCR (A, B) and western blot (C, D) were used to measure DCLRE1B and HOMER1 expressions.| Application Notes | Western Blot: Use at 1:1000 ~ 2000.Optimal working concentrations should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Can be stored at +4C. For long term storage, aliquot and store at -20C. Avoid repeated freezing and thawing cycles. |
| Storage Buffer | Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide. |
