Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HP1α (Phospho-Ser92) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | HP1 alpha (phospho Ser92) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HP1 alpha (phospho Ser92)This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide sequence around phosphorylation site of Serine 92(R-S-S(p)-N-F) derived from Human HP1α . |
| Modification | p-Ser92 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| IHC: 1:50 - 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HP1A HP1 CBX5 chromobox homolog 5 |
|---|---|
| Target | HP1 alpha (phospho Ser92) |
| Tissue Specificity | The antibody detects endogenous levels of HP1α only when phosphorylated at serine 92. |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HP1α (Phospho-Ser92) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
