Application
WB analysis of extracts from various cell lines using HP1 alpha antibody.Application
IHC-P analysis of human lung cancer using HP1 alpha antibody at a dilution of 1:200.Application
IHC-P analysis of human stomach cancer using HP1 alpha antibody at a dilution of 1:200.Application
ICC/IF analysis of HeLa cells using HP1 alpha antibody (green) and DAPI (blue).| Product Name | HP1 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HP1 alphaThis gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human CBX5 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBChIP |
| WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200. IP: 1:50 - 1:200. ChIP: 1:20 - 1:100.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HP1A HP1 chromobox homolog 5 CBX5 |
|---|---|
| Target | HP1 alpha |
| Molecular Weight(MW) | 22 kDa (note) |
Application
WB analysis of extracts from various cell lines using HP1 alpha antibody.
Application
IHC-P analysis of human lung cancer using HP1 alpha antibody at a dilution of 1:200.
Application
IHC-P analysis of human stomach cancer using HP1 alpha antibody at a dilution of 1:200.
Application
ICC/IF analysis of HeLa cells using HP1 alpha antibody (green) and DAPI (blue).| Application Notes | WB: 1:500 - 1:2000. IHC: 1:50 - 1:200. IF: 1:50 - 1:200. IP: 1:50 - 1:200. ChIP: 1:20 - 1:100.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
