Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000Application
Western Blot analysis of HPCAL1 expression in transfected 293T cell line by HPCAL1 polyclonal antibody. A: Non-transfected lysate. B: HPCAL1 transfected lysate. 12% SDS PAGE diluted at 1:500| Product Name | HPCAL1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HPCAL1 (hippocalcin-like 1)The protein encoded by this gene is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. There are two alternatively spliced transcript variants of this gene, with multiple polyadenylation sites. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HPCAL1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Zebrafish, Xenopus laevis, Pig, Chicken, Sheep, Rhesus Monkey, Chimpanzee, BovineRat (98%), Zebrafish (90%), Xenopus laevis (96%), Pig (99%), Chicken (98%), Sheep (99%), Rhesus Monkey (99%), Chimpanzee (99%), Bovine (99%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BDR1 antibody HLP2 antibody VILIP-3 antibody HPCAL1 antibody calcium-binding protein BDR-1 antibody hippocalcin-like protein 1 antibody visinin-like protein 3 antibody hippocalcin-like 1 antibody |
|---|---|
| Molecular Weight(MW) | 22 kDa (note) (5) |
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000
Application
Western Blot analysis of HPCAL1 expression in transfected 293T cell line by HPCAL1 polyclonal antibody. A: Non-transfected lysate. B: HPCAL1 transfected lysate. 12% SDS PAGE diluted at 1:500| Positive Control | HPCAL1 tansfected 293T cell , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
