Application
HPS3 antibody [C3], C-term detects HPS3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HPS3 protein stained by HPS3 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33343 staining.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HPS3 antibody at 1:500 dilution.Application
Sample(30 μg of whole cell lysate) A:293T B:A431 C:H1299 D:HeLa S3 E:Hep G2 F:MOLT4 G:Raji 7.5% SDS PAGE diluted at 1:1000| Product Name | HPS3 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HPS3 (Hermansky-Pudlak syndrome 3)This gene encodes a protein containing a potential clathrin-binding motif, consensus dileucine signals, and tyrosine-based sorting signals for targeting to vesicles of lysosomal lineage. The encoded protein may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. Mutations in this gene are associated with Hermansky-Pudlak syndrome type 3. Alternate splice variants exist, but their full length sequence has not been determined. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HPS3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Pig, BovinePig (92%), Bovine (92%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686F0413 antibody FLJ22704 antibody SUTAL antibody HPS3 antibody Hermansky-Pudlak syndrome 3 protein antibody Hermansky-Pudlak syndrome 3 antibody |
|---|---|
| Molecular Weight(MW) | 114 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
HPS3 antibody [C3], C-term detects HPS3 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HPS3 protein stained by HPS3 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HPS3 antibody at 1:500 dilution.
Application
Sample(30 μg of whole cell lysate) A:293T B:A431 C:H1299 D:HeLa S3 E:Hep G2 F:MOLT4 G:Raji 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
