Application
Western blot experiment using HSBP1 Antibody at 1:90000 dilution to detect 100ng of 13kDa recombinant HSBP1 protein antigen.| Product Name | HSBP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSBP1 (heat shock factor binding protein 1)The heat-shock response is elicited by exposure of cells to thermal and chemical stress and through the activation of HSFs (heat shock factors) results in the elevated expression of heat-shock induced genes. Heat shock factor binding protein 1 (HSBP1), is a 76-amino-acid protein that binds to heat shock factor 1(HSF1), which is a transcription factor involved in the HS response. During HS response, HSF1 undergoes conformational transition from an inert non-DNA-binding monomer to active functional trimers. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulate HSF1 DNA-binding activity. Overexpression of HSBP1 in mammalian cells represses the transactivation activity of HSF1. When overexpressed in C.elegans HSBP1 has severe effects on survival of the animals after thermal and chemical stress consistent with a role of HSBP1 as a negative regulator of heat shock response. [provided by RefSeq] |
| Immunogen | Full length human HSBP1 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Predicted Reactivity | Human, Mouse, Rat, Xenopus laevis, Pig, Chicken, Rhesus Monkey, BovineHuman (100%), Mouse (88%), Rat (88%), Xenopus laevis (82%), Pig (96%), Chicken (90%), Rhesus Monkey (98%), Bovine (98%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686D1664 antibody DKFZp686O24200 antibody NPC-A-13 antibody HSBP1 antibody heat shock factor-binding protein 1 antibody nasopharyngeal carcinoma-associated antigen 13 antibody heat shock factor binding protein 1 antibody |
|---|---|
| Molecular Weight(MW) | 9 kDa (note) |
| Cellular Localization | Nucleus |
Application
Western blot experiment using HSBP1 Antibody at 1:90000 dilution to detect 100ng of 13kDa recombinant HSBP1 protein antigen.| Positive Control | Target recombinant protein |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
