Application
HSD11B1 antibody detects HSD11B1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSD11B1 protein stained by HSD11B1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.Application
HSD11B1 antibody detects HSD11B1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. HSD11B1 antibody dilution: 1:500.Application
Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE diluted at 1:1000| Product Name | HSD11B1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSD11B1 (hydroxysteroid (11-beta) dehydrogenase 1)The protein encoded by this gene is a microsomal enzyme that catalyzes the conversion of the stress hormone cortisol to the inactive metabolite cortisone. In addition, the encoded protein can catalyze the reverse reaction, the conversion of cortisone to cortisol. Too much cortisol can lead to central obesity, and a particular variation in this gene has been associated with obesity and insulin resistance in children. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human HSD11B1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanSheep |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 11-DH antibody 11-beta-HSD1 antibody HDL antibody HSD11 antibody HSD11B antibody HSD11L antibody MGC13539 antibody SDR26C1 antibody HSD11B1 antibody corticosteroid 11-beta-dehydrogenase isozyme 1 antibody short chain dehydrogenase/reductase famil |
|---|---|
| Molecular Weight(MW) | 32 kDa (note) (3) |
| Cellular Localization | Endoplasmic reticulum membrane |
Application
HSD11B1 antibody detects HSD11B1 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSD11B1 protein stained by HSD11B1 antibody diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] diluted at 1:10000. Blue: Hoechst 33342 staining.
Application
HSD11B1 antibody detects HSD11B1 protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma. HSD11B1 antibody dilution: 1:500.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE diluted at 1:1000| Positive Control | HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
