Application
Sample (30 µg of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
HSD17B3 antibody detects HSD17B3 protein at cytoplasm by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: HSD17B3 protein stained by HSD17B3 antibody diluted at 1:500. Blue: Hoechst 33342 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using HSD17B3 antibody antibody at 1:500 dilution.| Product Name | HSD17B3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSD17B3 (hydroxysteroid (17-beta) dehydrogenase 3)This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSD17B3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Pig, Rhesus Monkey, BovinePig (80%), Rhesus Monkey (97%), Bovine (82%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | EDH17B3 antibody SDR12C2 antibody HSD17B3 antibody short chain dehydrogenase/reductase family 12C member 2 antibody 17-beta-HSD3 antibody testicular 17-beta-hydroxysteroid dehydrogenase antibody 17-beta-hydroxysteroid dehydrogenase type 3 antibody |
|---|---|
| Molecular Weight(MW) | 35 kDa (note) (2) |
Application
Sample (30 µg of whole cell lysate) A: Raji 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
HSD17B3 antibody detects HSD17B3 protein at cytoplasm by confocal immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: HSD17B3 protein stained by HSD17B3 antibody diluted at 1:500. Blue: Hoechst 33342 staining. [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using HSD17B3 antibody antibody at 1:500 dilution.| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
