Application
HSD3a antibody [N1C2] detects HSD3a protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSD3a protein stained by HSD3a antibody [N1C2] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using AKR1C4 antibody at 1:100 dilution.Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE HSD3a antibody diluted at 1:1000| Product Name | HSD3a antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to HSD3a (aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4))This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human HSD3a. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (81%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 3-alpha-HSD antibody C11 antibody CDR antibody CHDR antibody DD4 antibody HAKRA antibody MGC22581 antibody AKR1C4 antibody dihydrodiol dehydrogenase isozyme DD4 antibody type I 3-alpha-hydroxysteroid dehydrogenase antibody 3-alpha-HSD1 antibody |
|---|---|
| Target | HSD3a |
| Molecular Weight(MW) | 37 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
HSD3a antibody [N1C2] detects HSD3a protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: HSD3a protein stained by HSD3a antibody [N1C2] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using AKR1C4 antibody at 1:100 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE HSD3a antibody diluted at 1:1000| Positive Control | HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
