Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 (Phospho-Ser303) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from MCF7 cells using HSF1 (phospho-Ser303) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).| Product Name | HSF1 (phospho Ser303) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to HSF1 (Phospho Ser303)DNA-binding protein that specifically binds heat shock promoter elements (HSE) and activates transcription. In higher eukaryotes, HSF is unable to bind to the HSE unless the cells are heat shocked. |
| Immunogen | Peptide sequence around phosphorylation site of serine 303 (P-P-S(p)-P-P) derived from Human HSF1. |
| Modification | p-Ser303 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HSTF1 HSF1 HSTF 1 heat shock factor protein 1 HSF 1 heat shock transcription factor 1 |
|---|---|
| Target | HSF1 (Phospho Ser303) |
| Molecular Weight(MW) | 82 (note) |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 (Phospho-Ser303) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from MCF7 cells using HSF1 (phospho-Ser303) Antibody (Lane 2) and the same antibody preincubated with blocking peptide (Lane1).| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
